English
norskBlar i forfatter "Grgic, Miriam"
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Aliivibrio wodanis as a production host: development of genetic tools for expression of cold-active enzymes
(Journal article; Tidsskriftartikkel; Peer reviewed, 2019-11-11)<i>Background</i>: Heterologous production of cold-adapted proteins currently represents one of the greatest bottlenecks in the ongoing bioprospecting eforts to fnd new enzymes from low-temperature environments, such as, the polar oceans that represent essentially untapped resources in this respect. In mesophilic expression hosts such as <i>Escherichia coli</i>, cold-adapted enzymes often form ... -
Biochemical characterization of ParI, an orphan C5-DNA methyltransferase from Psychrobacter arcticus 273-4
(Journal article; Tidsskriftartikkel; Peer reviewed, 2018-05-25)<p>Cytosine-specific DNA methyltransferases are important enzymes in most living organisms. In prokaryotes, most DNA methyltransferases are members of the type II restriction-modification system where they methylate host DNA, thereby protecting it from digestion by the accompanying restriction endonucleases. DNA methyltransferases can also act as solitary enzymes having important roles in controlling ... -
DNA binding with a minimal scaffold: structure-function analysis of Lig E DNA ligases
(Journal article; Tidsskriftartikkel; Peer reviewed, 2018-07-11)DNA ligases join breaks in the phosphodiester backbone of DNA by catalysing the formation of bonds between opposing 5′P and 3′OH ends in an adenylation-dependent manner. Catalysis is accompanied by reorientation of two core domains to provide access to the active site for cofactor utilization and enable substrate binding and product release. The general paradigm is that DNA ligases engage their DNA ... -
Effects of Interventions to Prevent Work-Related Asthma, Allergy, and Other Hypersensitivity Reactions in Norwegian Salmon Industry Workers (SHInE): Protocol for a Pragmatic Allocated Intervention Trial and Related Substudies
(Journal article; Tidsskriftartikkel; Peer reviewed, 2023-05-07)Background: Workers in the salmon processing industry have an increased risk of developing respiratory diseases and other hypersensitivity responses due to occupational exposure to bioaerosols containing fish proteins and microorganisms, and related allergens. Little is known about effective measures to reduce bioaerosol exposure and about the extent of skin complaints among workers. In addition, ... -
Insights into bacterial protection and survival. A study of three enzymes from cold-adapted bacteria
(Doctoral thesis; Doktorgradsavhandling, 2015-10-02)Bacteria are the most abundant organisms and can be found in different habitats, from polar regions, deserts and volcanoes, deep ocean trenches to the upper atmosphere. In all these environments, they are exposed to various chemical hazards, such as antibacterial chemicals (antibiotics) produced by other organisms that are found in the same habitat. In addition, they are exposed to threats from ... -
Properties and distribution of a metallo-β-lactamase (ALI-1) from the fish pathogen Aliivibrio salmonicida LFI1238
(Journal article; Tidsskriftartikkel; Peer reviewed, 2015)Objectives. To characterize the chromosome-encoded metallo-β-lactamase (MBL) from the psychrophilic, marine fish-pathogenic bacterium Aliivibrio salmonicida LFI1238 and check for the presence of the gene in other Aliivibrio isolates both connected to the fish-farming industry and from the environment. Methods. The MBL gene was cloned and intracellularly expressed in Escherichia coli. Kinetic ...
